cd69 pe Search Results


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phycoerythrin pe conjugated cd69  (R&D Systems)
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cd69  (Miltenyi Biotec)
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cd69 pe rea824  (Miltenyi Biotec)
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cd69 h1 2 f3  (Cytek Biosciences)
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anti cd69 pe cy7  (Cytek Biosciences)
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(A) Mice were immunized i.n. with OVA-containing formulations on d0 and lungs and spleens were analyzed on d30 or d60 via tetramer and surface marker staining. CXCR3 was used as a marker of AW residence; CD103 and <t>CD69</t> were used as markers of tissue residency. (B-E) Number (#) and frequency (%) of Tet+ CD8+ T cells in (B) AW, (C) IST, (D) MV, and (E) spleen were enumerated on d30 after i.n. administration of OVA-NP/CpG, OVA-NP, or OVA+CpG. (F-I) Number (#) and frequency (%) of Tet+ CD8+ T cells in (F) AW, (G) IST, (H) MV, and (I) spleen were enumerated on d60 after i.n. administration of OVA-NP/CpG, OVA-NP, or OVA+CpG. (J) Flow cytometry was used to quantify Tet+ CD8+ T cells expressing TRM markers (CD103, CD69) in the airway (CXCR3hi) and lung interstitium (CXCR3lo). (K-L) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in AW were enumerated on (K) d30 or (L) d60 after immunization. (M-N) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in IST were enumerated on (M) d30 or (N) d60 after immunization. Data are mean ± SEM and representative of two to four independent experiments, with n = 5–6 per group. Immunization dose: 25 μg NP, 7.5 μg OVA, 1.4 μg. CpG Limit of detection: 1 cell (AW), 5 cells (IST/MV), 25 cells (spleen). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, by (B-I) ordinary one-way ANOVA or (K-N) ordinary two-way ANOVA with Tukey’s multiple comparisons test. ns, not significant. Statistical comparisons are shown for OVA-NP/CpG only.
Anti Cd69 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd69  (Miltenyi Biotec)
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(A) Mice were immunized i.n. with OVA-containing formulations on d0 and lungs and spleens were analyzed on d30 or d60 via tetramer and surface marker staining. CXCR3 was used as a marker of AW residence; CD103 and <t>CD69</t> were used as markers of tissue residency. (B-E) Number (#) and frequency (%) of Tet+ CD8+ T cells in (B) AW, (C) IST, (D) MV, and (E) spleen were enumerated on d30 after i.n. administration of OVA-NP/CpG, OVA-NP, or OVA+CpG. (F-I) Number (#) and frequency (%) of Tet+ CD8+ T cells in (F) AW, (G) IST, (H) MV, and (I) spleen were enumerated on d60 after i.n. administration of OVA-NP/CpG, OVA-NP, or OVA+CpG. (J) Flow cytometry was used to quantify Tet+ CD8+ T cells expressing TRM markers (CD103, CD69) in the airway (CXCR3hi) and lung interstitium (CXCR3lo). (K-L) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in AW were enumerated on (K) d30 or (L) d60 after immunization. (M-N) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in IST were enumerated on (M) d30 or (N) d60 after immunization. Data are mean ± SEM and representative of two to four independent experiments, with n = 5–6 per group. Immunization dose: 25 μg NP, 7.5 μg OVA, 1.4 μg. CpG Limit of detection: 1 cell (AW), 5 cells (IST/MV), 25 cells (spleen). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, by (B-I) ordinary one-way ANOVA or (K-N) ordinary two-way ANOVA with Tukey’s multiple comparisons test. ns, not significant. Statistical comparisons are shown for OVA-NP/CpG only.
Anti Cd69, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Immunity

Article Title: Low-Avidity CD4 + T Cell Responses to SARS-CoV-2 in Unexposed Individuals and Humans with Severe COVID-19

doi: 10.1016/j.immuni.2020.11.016

Figure Lengend Snippet:

Article Snippet: CD69-PE (REA824) , Miltenyi Biotec , Cat#130-112-613; RRID: AB_2659065.

Techniques: Functional Assay, Recombinant, Staining, Sequencing, Gene Expression, Software

(A) Mice were immunized i.n. with OVA-containing formulations on d0 and lungs and spleens were analyzed on d30 or d60 via tetramer and surface marker staining. CXCR3 was used as a marker of AW residence; CD103 and CD69 were used as markers of tissue residency. (B-E) Number (#) and frequency (%) of Tet+ CD8+ T cells in (B) AW, (C) IST, (D) MV, and (E) spleen were enumerated on d30 after i.n. administration of OVA-NP/CpG, OVA-NP, or OVA+CpG. (F-I) Number (#) and frequency (%) of Tet+ CD8+ T cells in (F) AW, (G) IST, (H) MV, and (I) spleen were enumerated on d60 after i.n. administration of OVA-NP/CpG, OVA-NP, or OVA+CpG. (J) Flow cytometry was used to quantify Tet+ CD8+ T cells expressing TRM markers (CD103, CD69) in the airway (CXCR3hi) and lung interstitium (CXCR3lo). (K-L) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in AW were enumerated on (K) d30 or (L) d60 after immunization. (M-N) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in IST were enumerated on (M) d30 or (N) d60 after immunization. Data are mean ± SEM and representative of two to four independent experiments, with n = 5–6 per group. Immunization dose: 25 μg NP, 7.5 μg OVA, 1.4 μg. CpG Limit of detection: 1 cell (AW), 5 cells (IST/MV), 25 cells (spleen). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, by (B-I) ordinary one-way ANOVA or (K-N) ordinary two-way ANOVA with Tukey’s multiple comparisons test. ns, not significant. Statistical comparisons are shown for OVA-NP/CpG only.

Journal: ACS nano

Article Title: Mucosal Immunization with a pH-Responsive Nanoparticle Vaccine Induces Protective CD8 + Lung-Resident Memory T Cells

doi: 10.1021/acsnano.9b00326

Figure Lengend Snippet: (A) Mice were immunized i.n. with OVA-containing formulations on d0 and lungs and spleens were analyzed on d30 or d60 via tetramer and surface marker staining. CXCR3 was used as a marker of AW residence; CD103 and CD69 were used as markers of tissue residency. (B-E) Number (#) and frequency (%) of Tet+ CD8+ T cells in (B) AW, (C) IST, (D) MV, and (E) spleen were enumerated on d30 after i.n. administration of OVA-NP/CpG, OVA-NP, or OVA+CpG. (F-I) Number (#) and frequency (%) of Tet+ CD8+ T cells in (F) AW, (G) IST, (H) MV, and (I) spleen were enumerated on d60 after i.n. administration of OVA-NP/CpG, OVA-NP, or OVA+CpG. (J) Flow cytometry was used to quantify Tet+ CD8+ T cells expressing TRM markers (CD103, CD69) in the airway (CXCR3hi) and lung interstitium (CXCR3lo). (K-L) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in AW were enumerated on (K) d30 or (L) d60 after immunization. (M-N) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in IST were enumerated on (M) d30 or (N) d60 after immunization. Data are mean ± SEM and representative of two to four independent experiments, with n = 5–6 per group. Immunization dose: 25 μg NP, 7.5 μg OVA, 1.4 μg. CpG Limit of detection: 1 cell (AW), 5 cells (IST/MV), 25 cells (spleen). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, by (B-I) ordinary one-way ANOVA or (K-N) ordinary two-way ANOVA with Tukey’s multiple comparisons test. ns, not significant. Statistical comparisons are shown for OVA-NP/CpG only.

Article Snippet: In experiments evaluating tissue-resident memory markers on d30 and d60, cells from lungs and spleens were also stained with anti-CD69-PE/Cy7 (clone H1.2F3; Tonbo), anti-CD103-Brilliant Violet 510 (clone 2E7; BioLegend), and anti-CXCR3-PerCP/Cy5.5 (clone CXCR3–173; BioLegend).

Techniques: Marker, Staining, Flow Cytometry, Expressing

(A) Mice were immunized i.n. with Flu-containing formulations on d0 and lungs and spleens were analyzed on d30 or d60 via Tet and surface marker staining. CXCR3 was used as a marker of AW residence; CD103 and CD69 were used as markers of tissue residency. (B-E) Number (#) and frequency (%) of Tet+ CD8+ T cells in (B) AW, (C) IST, (D) MV, and (E) spleen were enumerated on d30 after i.n. administration of Flu-NP/CpG, Flu-NP, or Flu+CpG. (F-I) Number (#) and frequency (%) of Tet+ CD8+ T cells in (F) AW, (G) IST, (H) MV, and (I) spleen were enumerated on d60 after i.n. administration of Flu-NP/CpG, Flu-NP, or Flu+CpG. (J-M) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in AW were enumerated on (J) d30 or (K) d60 after immunization. (L-M) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in IST were enumerated on (L) d30 or (M) d60 after immunization. Data are mean ± SEM, with n = 3–6 per group, and representative of two independent experiments. Immunization dose: 25 μg NP, 9.5 μg Flu, 1.4 μg CpG. Limit of detection: 1 cell (AW), 5 cells (IST/MV), 25 cells (spleen). *p<0.05, **p<0.01, ***p<0.001, by (B-I) ordinary one-way ANOVA or (J-M) ordinary two-way ANOVA with Tukey’s multiple comparisons test. ns, not significant.

Journal: ACS nano

Article Title: Mucosal Immunization with a pH-Responsive Nanoparticle Vaccine Induces Protective CD8 + Lung-Resident Memory T Cells

doi: 10.1021/acsnano.9b00326

Figure Lengend Snippet: (A) Mice were immunized i.n. with Flu-containing formulations on d0 and lungs and spleens were analyzed on d30 or d60 via Tet and surface marker staining. CXCR3 was used as a marker of AW residence; CD103 and CD69 were used as markers of tissue residency. (B-E) Number (#) and frequency (%) of Tet+ CD8+ T cells in (B) AW, (C) IST, (D) MV, and (E) spleen were enumerated on d30 after i.n. administration of Flu-NP/CpG, Flu-NP, or Flu+CpG. (F-I) Number (#) and frequency (%) of Tet+ CD8+ T cells in (F) AW, (G) IST, (H) MV, and (I) spleen were enumerated on d60 after i.n. administration of Flu-NP/CpG, Flu-NP, or Flu+CpG. (J-M) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in AW were enumerated on (J) d30 or (K) d60 after immunization. (L-M) Number (#) of Tet+ CD8+ T cells expressing CD69±CD103 in IST were enumerated on (L) d30 or (M) d60 after immunization. Data are mean ± SEM, with n = 3–6 per group, and representative of two independent experiments. Immunization dose: 25 μg NP, 9.5 μg Flu, 1.4 μg CpG. Limit of detection: 1 cell (AW), 5 cells (IST/MV), 25 cells (spleen). *p<0.05, **p<0.01, ***p<0.001, by (B-I) ordinary one-way ANOVA or (J-M) ordinary two-way ANOVA with Tukey’s multiple comparisons test. ns, not significant.

Article Snippet: In experiments evaluating tissue-resident memory markers on d30 and d60, cells from lungs and spleens were also stained with anti-CD69-PE/Cy7 (clone H1.2F3; Tonbo), anti-CD103-Brilliant Violet 510 (clone 2E7; BioLegend), and anti-CXCR3-PerCP/Cy5.5 (clone CXCR3–173; BioLegend).

Techniques: Marker, Staining, Expressing